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Chlorophyll A vs Chlorophyll B: Core Differences

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How Chlorophyll A and B Affect Photosynthesis and Plant Health

Green plants and certain algae survive by producing energy from sunlight, water, and carbon dioxide through photosynthesis. In this process, light energy is transformed into chemical energy. In photosynthesis, water, and carbon dioxide, are converted into glucose, in the presence of sunlight; and oxygen is released into the environment. Besides playing a vital role in sustaining the life of plants and other organisms, photosynthesis supports the whole world by regulating oxygen and carbon dioxide levels. Photosynthesis sustains plant life, which in turn, is essential to maintain the food chains and food webs.


When you look into the process of photosynthesis, you will see that the primary pigment that facilitates the process is the green pigment called chlorophyll. This pigment is found in certain microorganisms like cyanobacteria and inside thylakoid membranes of chloroplasts of plant cells. These pigments absorb the red and blue wavelength light rays from the sunlight, which is necessary to initiate the process of photosynthesis.


The chloroplasts contain various types of chlorophyll that contribute differently to the process of photosynthesis. These types of chlorophyll are as follows.

  • Chlorophyll A- It is the most abundant type of chlorophyll, which absorbs light rays of wavelengths of 429 nm and 659 nm most effectively.

  • Chlorophyll B- It is a type of accessory pigment responsible for passing on light energy to chlorophyll a. It is found in plants and green algae. It absorbs the light rays of wavelengths 455 nm and 642 nm.

  • Chlorophyll C- This chlorophyll pigment is found predominantly in marine algae and absorbs light rays of wavelength 447-452 nm.

  • Chlorophyll D- This pigment is mainly found in cyanobacteria, and they absorb light rays of wavelengths outside the optical range such as 710 nm.

Both chlorophyll a and b play an imperative role in photosynthesis in plants. The primary pigment responsible for photosynthesis is chlorophyll a, and b absorbs sunlight to pass it on to chlorophyll a.


The difference between chlorophyll a and chlorophyll b is tabulated below.


Difference Between Chlorophyll A and B

Chlorophyll A

Chlorophyll B

It is the principal pigment involved in photosynthesis.

It is an accessory pigment that helps in photosynthesis.

All plants, algae, bacteria, cyanobacteria, and phototrophs contain chlorophyll a.

Chlorophyll b is only present in green plants and algae.

It absorbs a range of violet-blue light and orange-red light from sunlight.

It only absorbs orange-red light from sunlight.

The absorption range of light is 430 nm to 660 nm for chlorophyll a. Its rate of absorption is also high.

The light rays of wavelengths 450 nm to 650 nm are absorbed by chlorophyll b. The rate of absorption is low.

Chlorophyll a absorbs red wavelengths more.

Chlorophyll b absorbs violet-blue light.

Chlorophyll a reflects blue-green light.

Chlorophyll b reflects orange-red light.

The chlorine ring of Chlorophyll-a has a methyl group in the third position.

In the chlorophyll b structure, there is an aldehyde attached to the chlorine ring in the third position.

Its structure makes it soluble in petroleum ether. It also has low solubility in polar solvents.

The structure of chlorophyll b makes it soluble in methyl alcohol. It has a high solubility in polar solvents.

It has a \[CH_{3}\] side group in the porphyrin ring.

It has a CHO group other than \[CH_{3}\] in the porphyrin ring.

The molecular formula of chlorophyll a is \[C_{55}H_{72}O_{5}N_{4}Mg\]. (Molecular weight is 839.51gmol).

The chlorophyll b formula is \[C_{55}H_{70}O_{6}N_{4}Mg\]. (Molecular weight is 907.49g/mol).

Almost 3/4th of chlorophyll found in plants is of this variant.

It accounts for only 1/4th of the chlorophyll present in plants. 

The core proteins’ antenna array reacts in chlorophyll a.

Chlorophyll b regulates the size of the antenna of the photosystem.


From the above table, Chlorophyll a vs. Chlorophyll b comparison must have become easier to understand.


The following diagram shows the structure of chlorophyll a and b and how both work within a photosystem.


(Image will be uploaded soon)


(Image will be uploaded soon)


Fun Facts About Chlorophyll

  • Chlorophyll is Good for Your Health

Many people consume chlorophyll as a health supplement, and medical studies suggest it has several health benefits. It is said to be beneficial in slowing signs of aging, treatment of acne, and fighting anemia of hemoglobin deficiency.

Studies also suggest that it helps fight cancer, remove body odour, and speed up the healing of wounds. However, it is unsafe to consume supplements without asking your doctor first, and further studies are going on in this field.

  • Animals That Use Photosynthesis

Apart from plants and microorganisms like algae, some animals have also utilized photosynthesis for additional energy. For instance, the oriental hornet uses a pigment called xanthopterin for converting solar energy into electricity. The spotted salamander has a symbiotic relationship with algae and uses photosynthesis for additional oxygen supply. A species of sea slug also steals chloroplasts from marine algae to carry out photosynthesis with its own cells.


Difference Between Chlorophyll A and Chlorophyll B

The above text is flooded with information related to chlorophyll a and chlorophyll b. It also stated the difference between them and some fun facts about it. 


Biology is a branch of science that deals with the study of living organisms. It is an essential course to study at both school and college levels. Some students find biology tough but it doesn't have to be like that. Getting good results in biology means looking at some of the major issues a person will face and having tips to deal with them. 


Below mentioned are a few tips and tricks which will provide you with all the understanding of the concepts and that too with not much effort.


Tips and strategies to improve your skill in biology are as follows:

Come to class with prior knowledge 

Reading from the textbook or copying down someone else's notes is not enough to make up for missed classes. Biology is a demanding subject and it needs more attention, understanding, and effort. Students are required to thoroughly read the study material, focus more on understanding every bit of the syllabus and then, ensure that whatever they have learnt and understood helps them to fetch marks. 

  • Hands-on experiments instead of catching up

Most of the knowledge in biology depends upon hands-on experimentation instead of catching up. The key to learning biology is to complete all the assignments and get well-versed in the subject matter on time. Hence, students are required to focus on experiments too. Completing the theory part would not give you the desired results and also, it would affect your subject knowledge. Therefore, make sure that you devote enough time to both the parts and focus on understanding the concepts better. 

  • Proceed from general to specific 

Another important point that students shall keep in mind before processing with revising or learning the subject is that Biology is a lot different than maths as it contains in-depth biological mechanisms and processes. Therefore, one must focus on all concepts before taking specific ones. Students shall also make sure to understand every bit of the concept with utmost sincerity because these concepts would later come up with specific details and if you don't make yourself clear with the basics, you would even struggle to grasp the new specific ones. 

  • Giving priority to lab time 

Practicality always gives us more experimental knowledge than theory subjects. One needs to practice biology in labs and it is one of the most effective ways to gather knowledge in biology. Students shall remember that as much as it is important to be thorough with the theory portion of the subject, it is also important that you focus on practical learning because that is where you get to apply your classroom learning and concepts. 

  • Drawings and Diagrams

Using imagery such as drawings and labeled diagrams in biology helps to understand biology more readily. Since it is one of the most effective ways of learning, it helps you to understand better, memorize it and also, retain things for a longer period of time. Hence, students are advised to use as many drawings, flowcharts, diagrams, and all other forms of representations of concepts; as possible so as to learn and understand better.

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FAQs on Chlorophyll A vs Chlorophyll B: Core Differences

1. What are the core differences between chlorophyll a and chlorophyll b?

The main differences between chlorophyll a and chlorophyll b lie in their function, structure, and the light they absorb.

  • Function: Chlorophyll a is the principal pigment that directly converts light energy into chemical energy. Chlorophyll b is an accessory pigment that captures light energy and transfers it to chlorophyll a.
  • Structure: Chlorophyll a has a methyl group (-CH₃) on its porphyrin ring, whereas chlorophyll b has an aldehyde group (-CHO) in the same position.
  • Colour and Absorption: Chlorophyll a appears blue-green and absorbs light most strongly in the violet-blue and orange-red parts of the spectrum. Chlorophyll b appears yellow-green and absorbs light most strongly in the blue and orange parts of the spectrum.

2. What is the key structural difference between chlorophyll a and chlorophyll b?

The key structural difference is the functional group attached to the third carbon position of the porphyrin ring. Chlorophyll a possesses a methyl group (-CH₃), while chlorophyll b has an aldehyde group (-CHO) at this location. This minor alteration is responsible for the different absorption spectra of the two molecules.

3. How do the absorption spectra of chlorophyll a and b differ?

Chlorophyll a and chlorophyll b absorb light most effectively at different wavelengths, which allows the plant to use a broader range of the light spectrum.

  • Chlorophyll a shows absorption peaks at approximately 430 nm (blue) and 662 nm (red).
  • Chlorophyll b shows absorption peaks at approximately 453 nm (blue-violet) and 642 nm (orange-red).

4. What are the primary functions of chlorophyll a and chlorophyll b in photosynthesis?

Chlorophyll a is the primary photosynthetic pigment and is essential for photosynthesis. Its main function is to act as the reaction centre where the actual conversion of light energy into chemical energy occurs. Chlorophyll b, on the other hand, acts as an accessory pigment. Its function is to broaden the range of light wavelengths that can be used for photosynthesis. It absorbs light and funnels the energy to the chlorophyll a molecule in the reaction centre.

5. What is the chemical formula for chlorophyll a and chlorophyll b?

The chemical formulas for these pigments are slightly different due to their structural variation:

  • The formula for chlorophyll a is C₅₅H₇₂O₅N₄Mg.
  • The formula for chlorophyll b is C₅₅H₇₀O₆N₄Mg.
The difference in hydrogen and oxygen atoms accounts for the methyl group in chlorophyll a versus the aldehyde group in chlorophyll b.

6. Why is chlorophyll a considered the primary photosynthetic pigment while chlorophyll b is an accessory pigment?

Chlorophyll a is considered the primary pigment because it is located in the reaction centre of the photosystems (PS I and PS II). It is the only pigment capable of getting photo-oxidised, meaning it can donate an excited electron to begin the electron transport chain, which is the core of light-dependent reactions. Chlorophyll b, located in the light-harvesting complex (antenna), can only absorb light energy and transfer it to chlorophyll a; it cannot initiate the photochemical event itself. Therefore, it plays a supporting or 'accessory' role.

7. If chlorophyll b is so good at absorbing light, why can't plants survive with only chlorophyll b?

A plant cannot survive with only chlorophyll b because it lacks the ability to convert captured light energy into chemical energy. This critical process, known as photochemical conversion, is exclusively performed by the specialised chlorophyll a molecule at the reaction centre. While chlorophyll b expands the range of light absorption, it is functionally incapable of initiating the electron flow required for making ATP and NADPH. It can only pass the absorbed energy to chlorophyll a.

8. How does the ratio of chlorophyll a to chlorophyll b change in plants adapted to sun versus shade?

The ratio of chlorophyll a to b is a classic example of plant adaptation to light conditions.

  • Sun plants, growing in high light intensity, have a higher ratio of chlorophyll a to b (typically around 3:1). They have enough light, so they invest more in the primary pigment for conversion.
  • Shade plants, growing in low light, have a lower ratio of chlorophyll a to b, meaning they have a relatively higher amount of chlorophyll b. This is an adaptation to capture a broader spectrum of the limited light that filters through the canopy, particularly the blue-green wavelengths that chlorophyll b absorbs well.

9. How do plants with red or purple leaves photosynthesise if they aren't green?

Plants with red or purple leaves still contain both chlorophyll a and b and perform photosynthesis efficiently. The green colour of the chlorophyll is simply masked by a high concentration of other pigments called anthocyanins (which are red/purple). These pigments absorb light in the blue-green wavelengths and can also protect the leaf from damage by intense sunlight. Underneath this colourful layer, the chlorophyll is still present and actively carrying out photosynthesis.

10. Besides chlorophyll a and b, what other types of pigments are important in photosynthesis?

In addition to chlorophylls, plants rely on other accessory pigments to absorb a wider range of light and for protection. The most important group is the carotenoids, which includes:

  • Carotenes: These are orange pigments (like in carrots) that absorb blue and green light.
  • Xanthophylls: These are yellow pigments that also absorb blue-green light.
Both types of carotenoids absorb light at wavelengths where chlorophylls are inefficient and transfer the energy to the reaction centre. They also play a vital photoprotective role by safely dissipating excess light energy that could otherwise damage the chlorophyll molecules.